Quantitative analysis of liquid Culture

Purpose: To Analyze liquid culture for the presence of the tryptamine alkaloids psilocin and psilocybin.

Results: No Traces found

Here at ITW, we’ve been hard at work behind the scenes, and we have some big news to share. It is our great pleasure to
invite you to join us in a new mycological movement: the liquid culture revolution.

Like the best stories, ours always begins and ends with a spore: it is both the genesis and the outcome. But all that in
between, the substance, the bulk, that is mycelium. One would not exist without the other.

Spores come in all different shapes, colors and sizes, and a lot can be deciphered by studying them alone. But the mycelium is where the fungus expresses its true character, how it explores, interacts and affects its world. The hyphal tip with its spitzenkörper, exuding enzymes and weaving its subtle yet unrivaled influence, that is where the magic happens. It is truly a sight to behold.

A single mushroom-producing species can comprise so many distinct expressions, like different characters in the same tale,that you wouldn’t even know they are related. When you spend as much time staring into a microscope as we do, you develop a curiosity and passion for finding those new and unusual varieties. But as much diversity as there is between various phenotypes of a given species, there is even more to discover among specific isolations, that is, specific genetic individuals. This is especially true of those varieties that are most often cultivated, as they have been subjected to so many generations of artificial selection, just like how dogs have come to take so many different forms.

While these isolated individual strains can be cloned indefinitely, until now there has been no legal way to share and study them for fear that they might surreptitiously produce certain substances. Thus the vast majority of the exchange of these precious and magnificently unique genetics has taken place in secret, and at great risk. Thankfully, no one can stop the spread of spores: they’re in the wind. But what if we could share mycelium freely too?

Over the past few years, the team at ITW has been developing an answer to this question, and that answer is liquid culture. We’ve determined that, by using a specific process, ingredients and technique, it is possible to consistently produce liquid culture full of healthy, thriving mycelium that contains no detectable traces of any controlled chemical compounds. We have a lot more work to do to exhaustively test, corroborate and publish the results, but our preliminary findings are conclusive, and we think this is just too important to keep to ourselves.

As the culmination of countless experiments in the lab, we selected several cultures from several different strains that we felt would best represent the variety of conditions in which our liquid culture might be found in the course of its production, transportation and storage. We then sent them to our friends at Flourish Labs to have them redundantly analyzed, alongside a control, for minute traces of psilocybin and psilocin, using high-performance liquid chromatography. No traces of any alkaloids were found. We’ve shared the results with you here.

For legal reasons, we cannot share all of the details of our process, nor do we encourage nor condone the production of
these substances. However, there isn’t any special “secret” or proprietary technique. The main insight is that tryptamine
synthesis only occurs in mycelium during and after the earliest stages of fructification, evinced by the telltale bluing that often coincides with the “knotting” stage just prior to primordia development. Oxygen also appears to play a key role in this process. Mycelium, when induced into dormancy and subjected to anaerobic conditions, suspends all metabolic activity. (An astute observer might find other breadcrumbs scattered along the way.)

Our legal team has also emphatically insisted that, just because we have obtained these results, this does not mean that
they remain valid for other people, processes and products. We therefore encourage anyone who is interested in exploring this new territory to conduct their own analysis, and so we have partnered with Flourish Labs to offer you a great deal on professional liquid culture and potency testing. We hope you’ll help us support this valuable service. Click Here To Visit FlourishLabs

We have a lot more work to do to further verify this evidence and publish our findings for peer review. More sensitive
quantitative analysis requires more sensitive machinery, and while restrictions are loosening, most of these machines are found in the laboratories of large institutions which do not have the standards or license to conduct these experiments. We are therefore working to help our partners acquire these resources, and make them accessible to us all.

In the meantime, we hope that these findings will bring reassurance and excitement to our beloved mycological community, and open new doors for the future of fungal genetics exchange. This is the liquid culture revolution, and we couldn’t be more excited to join this movement with you. So tell your friends, tell your fungus, and while you’re at it, go check out our unmatched selection of pure, vibrant, isolated mycelium under “Liquid Cultures” at InoculateTheWorld.com!