What is a Spore Print? What is a Spore Syringe? What is Liquid Culture?

Written by ITW

May 17, 2021

When working with mushroom spores, there are many different ways in which they can be used and stored safely. In this brief article we go over the differences between three of these storage methods.  

What is a spore print? Spore Prints are the product of placing gills of a mushroom onto a flat clean surface such as tin foil, paper, mylar, glass, and plastic dishes. The options are nearly endless, however some mediums make for easier removal of the spores than others. In this condition, spores can last decades if stored in an airtight container kept in a cool dark place. For microscopic viewing of spores, simply use a tool to transfer the spores to a glass slide where they can be easily viewed under a microscope. Spores prints will vary in size, shape, and color. It depends on the size of the mushroom cap that drops spores as to how large or small your print will be. A spore print as small as a dime contains millions of individual spores. Mushroom species are also a factor that determines the size and color of a print. Certain species with small caps leave small spore prints, while others with large caps will leave large spore prints. A single spore print can make anywhere from 5 to 100 10ml spore syringes.

Photo by: Drew C

Next we will discuss the most common method for using spores, the spore syringe. 

What is a spore syringe? A spore syringe can be made by taking a print and scraping the spores into a sterile container of distilled water; then simply pulling the mixed solution into a sterile syringe. To use, you will vigorously shake the syringe to distribute spores throughout the solution. Next you will place a drop of the solution onto a glass slide, and place a cover-slip on top. Now place the slide on your microscope stage and explore your spores. Take photos, measure spore size, and most of all – have fun with your new research kit. Spore syringes will vary in spore density dependent upon species. In this condition, spores can last many years when stored in an airtight container and kept in a cool dark place.

Photo by: Drew C

What is liquid culture? Liquid Culture (LC) is obtained by taking a germinated agar plate, and blending the contents into a sterile nutritive liquid solution. After allowing it to brew in its new ‘liquid home’, the liquid inoculant can be pulled into a sterile syringe. 

Live mycelium. Photo by: TBG


Live mycelium growing in a jar of potato dextrose (LC). Photo by: TipOfTheCapMushrooms

What’s your best option? 

The one that most suits your needs. When buying from a reputable vendor like InoculateTheWorld.Com, you can be confident that whichever medium you choose to use will perform to your expectations. Spore syringes are certainly the most common medium for users. Spore prints are great for making syringes, and viewing spores under a microscope. Experiment and find what works best for you! Thanks for reading and be sure to let us know if you enjoyed this article by leaving a comment below. 



Where you go for spores.

Explore it, learn something new. And where it’s legal, order it.

Costa Rica (Arenal Volcano) Spore Print

A unique Cubensis first collected by LJ on the Arenal Volcano in Costa Rica. These are the F2 grow by BAS. Expect great and unique variation from these. Third photo is of the original specimen collected from the south side of the volcano. These are truly a winner. Expect Umbonate caps, consistent flushes and a new strain for your library.

Tosahatchee Spore Print

Tosahatchee is a wild Psilocybe Cubensis originally collected by InoculateTheWest in the cattle fields of Central Florida. It's name Tosahatchee comes from the region it was collected. These spores come from the first domesticated grow of the wild specimen. Basidium Equilibrium found these to be an extremely rewardgin strain to work, and the potency to be beyond any Cubensis he's experienced (MORE POTENT THAN APE).  We are grateful and proud to present to you, Psilocybe Cubensis Tosahatchee. 

Golden Halo Spore Print

Ps. Cubensis that produces gold pigmented spores. One of the most sought after strains on the market, available to you now. 

Ganoderma Oregonense "Reishi" Slant

A wild culture of Ganoderma Oregonense, commonly known as "reishi". Reishi is recognized for both its unique growth variations as well as its medicinal benefits. 

PE7 Spore Swab

PE7 is an isolation of Penis Envy that was kept in circulation due to its unique growth (flower caps) and intense potency. One-of-a-kind PE.

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